Anti-Human TSC2 Polyclonal Antibody (HW726014)

Name: Anti-Human TSC2 Polyclonal Antibody
Brand: abinScience
SKU: HW726014
Price: 168 USD
Availability: InStock

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개요
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SDS

개요

카탈로그 번호

HW726014

설명

Anti-Human TSC2 Polyclonal Antibody (HW726014) is a rabbit polyclonal antibody detecting TSC2 in ELISA, IHC, WB. Suitable for Human, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human TSC2 (Asp1541-Asn1731).

타겟

Tuberous sclerosis 2 protein, TSC2, TSC4, Tuberin

정제

Purified by antigen affinity column.

등록번호

P49815

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Tuberin (TSC2) is a ~200 kDa protein. Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling.

1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553

2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141

3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888

4. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785

5. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059

6. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219

7. Menon, S. et al. (2014) Cell 156, 771-85. PMID: 24529379

8. Lim, JS. et al. (2017) American journal of human genetics 100, 454-472. PMID: 28215400

9. Yang, H. et al. (2021) Nature communications 12, 339. PMID: 33436626

10. Ali, ES. et al. (2022) Molecular cell 82, 3284-3298.e7. PMID: 35772404

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human TSC2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human TSC2 Polyclonal Antibody (HW726014)

개요

이미지

참고 문헌

데이터시트

SDS