Anti-Human U2AF2 Polyclonal Antibody (HB779014)

Name: Anti-Human U2AF2 Polyclonal Antibody
Brand: abinScience
SKU: HB779014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HB779014

설명

Anti-Human U2AF2 Polyclonal Antibody (HB779014) is a rabbit polyclonal antibody detecting U2AF2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human U2AF2 (Pro138-Gly338).

타겟

Splicing factor U2AF 65 kDa subunit, hU2AF65, hU2AF(65), U2 auxiliary factor 65 kDa subunit, U2AF65, U2AF2, U2 snRNP auxiliary factor large subunit

정제

Purified by antigen affinity column.

등록번호

P26368

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Splicing factor U2AF 65 kDa subunit (U2AF2) is a ~53 kDa protein. Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly.

1. Millevoi, S. et al. (2006) The EMBO journal 25, 4854-64. PMID: 17024186

2. David, CJ. et al. (2011) Genes & development 25, 972-83. PMID: 21536736

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-U2AF2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate

참고 문헌

데이터시트

Anti-Human U2AF2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human U2AF2 Polyclonal Antibody (HB779014)

개요

이미지

참고 문헌

데이터시트

SDS