Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

Name: Anti-Human UBE2D3 Polyclonal Antibody
Brand: abinScience
SKU: HX067014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HX067014

설명

Anti-Human UBE2D3 Polyclonal Antibody (HX067014) is a rabbit polyclonal antibody detecting UBE2D3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human UBE2D3 (Met1-Met147).

타겟

Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3

정제

Purified by antigen affinity column.

등록번호

P61077

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin.

1. Rajendra, R. et al. (2004) The Journal of biological chemistry 279, 36440-4. PMID: 15247280

2. Huang, J. et al. (2004) The Journal of biological chemistry 279, 54110-6. PMID: 15496420

3. Kubori, T. et al. (2008) Molecular microbiology 67, 1307-19. PMID: 18284575

4. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

5. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592

6. Peng, H. et al. (2017) Cell research 27, 657-674. PMID: 28322253

7. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421

8. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human UBE2D3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

개요

이미지

참고 문헌

데이터시트

SDS