Anti-Human UBR5 Polyclonal Antibody (HC972014)

Name: Anti-Human UBR5 Polyclonal Antibody
Brand: abinScience
SKU: HC972014
Price: 168 USD
Availability: InStock

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100ug

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개요
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개요

카탈로그 번호

HC972014

설명

Anti-Human UBR5 Polyclonal Antibody (HC972014) is a rabbit polyclonal antibody detecting UBR5. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human UBR5 (Pro640-Ala870).

타겟

E3 ubiquitin-protein ligase UBR5, E3 ubiquitin-protein ligase, HECT domain-containing 1, EC:2.3.2.26, EDD, EDD1, HYD, Hyperplastic discs protein homolog, KIAA0896, Progestin-induced protein, UBR5, hHYD

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

O95071

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

E3 ubiquitin-protein ligase UBR5 is a ~309 kDa protein. E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm and nucleus. Mainly acts as a ubiquitin chain elongator that extends pre-ubiquitinated substrates. Component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation. Recognizes type-1 N-degrons, containing positively charged amino acids (Arg, Lys and His). Together with UBR4, part of a cytoplasm protein quality control pathway that prevents protein aggregation by catalyzing assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, leading to substrate recognition by the segregase p97/VCP and degradation by the proteasome: UBR5 is probably branching multiple 'Lys-48'-linked chains of substrates initially modified with mixed conjugates by UBR4.

1. Yau, RG. et al. (2017) Cell 171, 918-933.e20. PMID: 29033132

2. Schukur, L. et al. (2020) Scientific reports 10, 20044. PMID: 33208877

3. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846

4. Mark, KG. et al. (2023) Cell 186, 3460-3475.e23. PMID: 37478862

5. Hodáková, Z. et al. (2023) The EMBO journal 42, e113348. PMID: 37409633

6. Ohtake, F. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1401-E1408. PMID: 29378950

10. Jiang, W. et al. (2011) Molecular cell 43, 33-44. PMID: 21726808

참고사항

For research use only

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참고 문헌

데이터시트

Anti-Human UBR5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human UBR5 Polyclonal Antibody (HC972014)

개요

이미지

참고 문헌

데이터시트

SDS

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