Anti-Human UNG Polyclonal Antibody (HY404014)

Name: Anti-Human UNG Polyclonal Antibody
Brand: abinScience
SKU: HY404014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD): --

사양:

50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY404014

설명

Anti-Human UNG Polyclonal Antibody (HY404014) is a rabbit polyclonal antibody detecting UNG. Suitable for Human.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human UNG (Ala45-Leu313).

타겟

DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P13051

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Uracil-DNA glycosylase (UNG) is a ~34 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication.

1. Imai, K. et al. (2003) Nature immunology 4, 1023-8. PMID: 12958596

2. Kavli, B. et al. (2005) The Journal of experimental medicine 201, 2011-21. PMID: 15967827

3. Akbari, M. et al. (2007) Neuroscience 145, 1201-12. PMID: 17101234

4. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144

5. Mol, CD. et al. (1995) Cell 82, 701-8. PMID: 7671300

6. Slupphaug, G. et al. (1996) Nature 384, 87-92. PMID: 8900285

7. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624

8. Bharati, S. et al. (1998) Nucleic acids research 26, 4953-9. PMID: 9776759

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-Human UNG Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human UNG Polyclonal Antibody (HY404014)

개요

이미지

참고 문헌

데이터시트

SDS