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| 카탈로그 번호 | HV097013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, IF, IP, IP-MS | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG | ||||
| 클론 ID | SAA0238 | ||||
| 타겟 | Ubiquitin-specific-processing protease 7, Deubiquitinating enzyme 7, Ubiquitin thioesterase 7, USP7, Herpesvirus-associated ubiquitin-specific protease, HAUSP, Ubiquitin carboxyl-terminal hydrolase 7 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | Q93009 | ||||
| RRID | Anti-Human USP7 IP Antibody (SAA0238) (abinScience Cat# HV097013, RRID:AB_3729772) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | Ubiquitin carboxyl-terminal hydrolase 7 (USP7) is a ~128 kDa protein. Hydrolase that deubiquitinates target proteins such as ARMC5, FOXO4, DEPTOR, KAT5, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, KMT2E/MLL5 and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. 1. Li, M. et al. (2002) Nature 416, 648-53. PMID: 11923872 2. Li, M. et al. (2004) Molecular cell 13, 879-86. PMID: 15053880 3. van der Horst, A. et al. (2006) Nature cell biology 8, 1064-73. PMID: 16964248 4. Song, MS. et al. (2008) Nature 455, 813-7. PMID: 18716620 5. Zhang, J. et al. (2014) Nature communications 5, 5059. PMID: 25283148 6. Cui, H. et al. (2015) Nature communications 6, 6752. PMID: 25865756 7. Ding, X. et al. (2015) PloS one 10, e0145023. PMID: 26678539 8. Jiang, L. et al. (2017) The Journal of biological chemistry 292, 13296-13311. PMID: 28655758 9. Yan, G. et al. (2021) Journal of cellular and molecular medicine 25, 3149-3159. PMID: 33544460 10. Vega, M. et al. (2022) The Journal of biological chemistry 298, 101750. PMID: 35216969 | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human USP7 IP Antibody (SAA0238)
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