Anti-Human Vimentin Recombinant Antibody (VB6) (HY550107)

Name: Anti-Human Vimentin Recombinant Antibody (VB6)
Brand: abinScience
SKU: HY550107
Price: 191 USD
Availability: InStock

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개요

카탈로그 번호	HY550107
종 반응성	Human
응용 분야	ELISA, FCM, WB
숙주 종	Human
클론성	Monoclonal
이소타입	IgG1, kappa
클론 ID	VB6
테스트된 응용 분야	FCM
타겟	Vimentin, VIM, RHC35301
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P08670
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경	Vimentin is a ~53 kDa protein. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips.
참고사항	For research use only.

이미지

Bioactivity

SEC-HPLC detection for Anti-Human VIM/Vimentin Antibody (VB6).
Flow Cytometry

Flow-cytometry using anti-human VIM antibody.VIM/Vimentin Transfected HI5 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human VIM antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VIM antibody monoclonal antibody (Catalog # HY550107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.