Anti-Human XRCC4 Polyclonal Antibody (HC509014)

Name: Anti-Human XRCC4 Polyclonal Antibody
Brand: abinScience
SKU: HC509014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HC509014

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human XRCC4 (Met1-Lys115).

타겟

DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4

정제

Purified by antigen affinity column.

등록번호

Q13426

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA repair protein XRCC4 is a ~38 kDa protein. DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination. Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors.

1. Nick McElhinny, SA. et al. (2000) Molecular and cellular biology 20, 2996-3003. PMID: 10757784

2. Chen, L. et al. (2000) The Journal of biological chemistry 275, 26196-205. PMID: 10854421

3. Lee, JW. et al. (2003) Cancer research 63, 22-4. PMID: 12517771

4. Foster, RE. et al. (2006) Biochemical and biophysical research communications 341, 175-83. PMID: 16412978

5. Mari, PO. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 18597-602. PMID: 17124166

6. Gu, J. et al. (2007) The EMBO journal 26, 1010-23. PMID: 17290226

7. Roy, S. et al. (2012) Nucleic acids research 40, 1684-94. PMID: 22228831

8. Wanotayan, R. et al. (2015) Biochemical and biophysical research communications 457, 526-31. PMID: 25597996

9. de Bruin, C. et al. (2015) The Journal of clinical endocrinology and metabolism 100, E789-98. PMID: 25742519

10. Fukuchi, M. et al. (2015) Biochemical and biophysical research communications 461, 687-94. PMID: 25934149

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human XRCC4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human XRCC4 Polyclonal Antibody (HC509014)

개요

이미지

참고 문헌

데이터시트

SDS

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