中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HY120014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human XRCC6 Polyclonal Antibody (HY120014) is a rabbit polyclonal antibody detecting XRCC6 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
|
||||||||
| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human XRCC6 (Asp36-Lys238). | ||||||||
| 타겟 | Lupus Ku autoantigen protein p70, 5'-dRP lyase Ku70, Thyroid-lupus autoantigen, XRCC6, CTC box-binding factor 75 kDa subunit, X-ray repair complementing defective repair in Chinese hamster cells 6, G22P1, X-ray repair cross-complementing protein 6, ATP-dependent DNA helicase 2 subunit 1, DNA repair protein XRCC6, TLAA, ATP-dependent DNA helicase II 70 kDa subunit, CTCBF, 70 kDa subunit of Ku antigen, Ku70, 5'-deoxyribose-5-phosphate lyase Ku70, CTC75 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P12956 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||||||
| 제품 사용 정보 |
| ||||||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | DNA repair protein Ku70 (XRCC6) is a ~69 kDa protein. DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with XRCC5 (Ku80), creating the Ku70:Ku80 heterodimer (Ku complex), which serves as a DNA end-binding complex. It primarily binds DSBs and recruits essential repair factors, assembling the core long-range NHEJ complex to facilitate the alignment and ligation of broken DNA ends. This pathway ensures the rapid repair of cytotoxic and mutagenic DSBs and contributes to the generation of diversity in T-cell receptors and antibodies through mechanisms such as V(D)J recombination. Likely acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), catalyzing the beta-elimination of the 5'-deoxyribose-5-phosphate at abasic sites near DSBs. 1. Walker, JR. et al. (2001) Nature 412, 607-14. PMID: 11493912 2. Liu, H. et al. (2010) Biochemical and biophysical research communications 397, 245-50. PMID: 20493174 3. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234 4. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203 5. West, RB. et al. (1998) Molecular and cellular biology 18, 5908-20. PMID: 9742108 6. Roberts, SA. et al. (2010) Nature 464, 1214-7. PMID: 20383123 7. Tuteja, N. et al. (1994) The EMBO journal 13, 4991-5001. PMID: 7957065 8. Maekawa, T. et al. (2018) Nucleic acids research 46, 4487-4504. PMID: 29490055 9. Willis, DM. et al. (2002) The Journal of biological chemistry 277, 37280-91. PMID: 12145306 10. Chung, U. et al. (1996) The Journal of biological chemistry 271, 8593-8. PMID: 8621488 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-XRCC6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Lncap cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: K562 cell lysate
Lane 6: 293T cell lysate
문의하기
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
