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Anti-Human YWHAG Polyclonal Antibody (HX994014)

Anti-Human YWHAG Polyclonal Antibody
Anti-Human YWHAG Polyclonal Antibody
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  • 개요

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개요
카탈로그 번호HX994014
설명
Anti-Human YWHAG Polyclonal Antibody (HX994014) is a rabbit polyclonal antibody detecting YWHAG in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human YWHAG (Met1-Asn247).
타겟 Protein kinase C inhibitor protein 1, 14-3-3 protein gamma, YWHAG, KCIP-1
정제 Purified by antigen affinity column.
등록번호 P61981
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

14-3-3 protein gamma (YWHAG) is a ~28 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24. Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1.

1. Yoshida, K. et al. (2005) Nature cell biology 7, 278-85. PMID: 15696159
2. Jin, Y. et al. (2006) The EMBO journal 25, 1207-18. PMID: 16511572
3. Dai, X. et al. (2023) Nature metabolism 5, 265-276. PMID: 36732624
4. Oh, SJ. et al. (2017) Molecular brain 10, 51. PMID: 29121962
5. Rietscher, K. et al. (2018) Journal of cell science 131. PMID: 29678907
참고사항 For research use only.
이미지
  • Anti-Human YWHAG Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-YWHAG polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: A431 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: A549 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: Jurkat cell lysate
    Lane 7: U251 cell lysate
    Lane 8: Mouse lung lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat testis lysate
    Lane11: Rat lung lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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