Anti-IFNAR2 Polyclonal Antibody (HW716014)

Name: Anti-IFNAR2 Polyclonal Antibody
Brand: abinScience
SKU: HW716014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HW716014

설명

Anti-IFNAR2 Polyclonal Antibody (HW716014) is a rabbit polyclonal antibody detecting IFNAR2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human IFNAR2 (Ile27-Lys243).

타겟

Interferon alpha binding protein, Interferon alpha/beta receptor 2, IFNAR2, IFN-alpha binding protein, IFN-R-2, IFNABR, IFNARB, Type I interferon receptor 2, IFN-alpha/beta receptor 2

정제

Purified by antigen affinity column.

등록번호

P48551

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Interferon alpha/beta receptor 2 (IFNAR2) is a ~57 kDa protein. Together with IFNAR1, forms the heterodimeric receptor for type I interferons (including interferons alpha, beta, epsilon, omega and kappa). Type I interferon binding activates the JAK-STAT signaling cascade, resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response. Mechanistically, type I interferon-binding brings the IFNAR1 and IFNAR2 subunits into close proximity with one another, driving their associated Janus kinases (JAKs) (TYK2 bound to IFNAR1 and JAK1 bound to IFNAR2) to cross-phosphorylate one another. The activated kinases phosphorylate specific tyrosine residues on the intracellular domains of IFNAR1 and IFNAR2, forming docking sites for the STAT transcription factors (STAT1, STAT2 and STAT). STAT proteins are then phosphorylated by the JAKs, promoting their translocation into the nucleus to regulate expression of interferon-regulated genes.

1. Russell-Harde, D. et al. (1999) Biochemical and biophysical research communications 255, 539-44. PMID: 10049744

2. Piehler, J. et al. (1999) Journal of molecular biology 294, 223-37. PMID: 10556041

3. Thomas, C. et al. (2011) Cell 146, 621-32. PMID: 21854986

4. Duncan, CJ. et al. (2015) Science translational medicine 7, 307ra154. PMID: 26424569

5. Arimoto, KI. et al. (2017) Nature structural & molecular biology 24, 279-289. PMID: 28165510

6. Zhang, Q. et al. (2020) Science (New York, N.Y.) 370. PMID: 32972995

7. Domanski, P. et al. (1995) The Journal of biological chemistry 270, 21606-11. PMID: 7665574

8. Novick, D. et al. (1995) Journal of leukocyte biology 57, 712-8. PMID: 7759950

9. Novick, D. et al. (1994) Cell 77, 391-400. PMID: 8181059

10. Platanias, LC. et al. (1996) The Journal of biological chemistry 271, 23630-3. PMID: 8798579

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-IFNAR2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-IFNAR2 Polyclonal Antibody (HW716014)

개요

이미지

참고 문헌

데이터시트

SDS