Anti-ILK Polyclonal Antibody (HC502014)

Name: Anti-ILK Polyclonal Antibody
Brand: abinScience
SKU: HC502014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HC502014

설명

Anti-ILK Polyclonal Antibody (HC502014) is a rabbit polyclonal antibody detecting ILK in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ILK (Asn183-Lys452).

타겟

ILK, ILK2, ILK1, ILK-2, ILK-1, 59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, p59ILK, Integrin-linked protein kinase

정제

Purified by antigen affinity column.

등록번호

Q13418

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Scaffold protein ILK is a ~51 kDa protein. Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration. Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation. Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration. Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion. Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes.

1. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

2. Fukuda, K. et al. (2009) Molecular cell 36, 819-30. PMID: 20005845

3. Vaynberg, J. et al. (2018) Nature communications 9, 4465. PMID: 30367047

4. Yang, L. et al. (2020) Nature 583, 133-138. PMID: 32528174

5. Yoshimi, R. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 3611-24. PMID: 16517730

6. Delcommenne, M. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 11211-6. PMID: 9736715

7. Fielding, AB. et al. (2008) The Journal of cell biology 180, 681-9. PMID: 18283114

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-ILK Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ILK Polyclonal Antibody (HC502014)

개요

이미지

참고 문헌

데이터시트

SDS