Anti-IRS1 Polyclonal Antibody (HW428014)

Name: Anti-IRS1 Polyclonal Antibody
Brand: abinScience
SKU: HW428014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HW428014

설명

Anti-IRS1 Polyclonal Antibody (HW428014) is a rabbit polyclonal antibody detecting IRS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human IRS1 (Pro4-Arg267).

타겟

IRS-1, IRS1, Insulin receptor substrate 1

정제

Purified by antigen affinity column.

등록번호

P35568

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Insulin receptor substrate 1 (IRS1) is a ~131 kDa protein. Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R. Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism. Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2. Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade. Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation.

1. Craparo, A. et al. (1995) The Journal of biological chemistry 270, 15639-43. PMID: 7541045

2. Yu, W. et al. (2021) The Journal of biological chemistry 296, 100739. PMID: 33991522

3. Woo, JR. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2401716121. PMID: 38625937

4. Longobardi, L. et al. (2009) Growth factors (Chur, Switzerland) 27, 309-20. PMID: 19639489

5. Tu, Y. et al. (2001) The Biochemical journal 354, 315-22. PMID: 11171109

6. Lee, CH. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11713-7. PMID: 8265614

7. Geng, Y. et al. (2014) The Journal of biological chemistry 289, 11230-11241. PMID: 24616100

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-IRS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-IRS1 Polyclonal Antibody (HW428014)

개요

이미지

참고 문헌

데이터시트

SDS

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