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| 카탈로그 번호 | HW621014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Ki67/MKI67 Polyclonal Antibody (HW621014) is a rabbit polyclonal antibody detecting MKI67 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human Ki67/MKI67 (Gly3088-Lys3235). | ||||||||
| 타겟 | MKI67, Proliferation marker protein Ki-67, Antigen Ki67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P46013 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Proliferation marker protein Ki-67 (Ki67/MKI67) is a ~358 kDa protein. Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis. Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface. The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space. 1. Cuylen, S. et al. (2016) Nature 535, 308-12. PMID: 27362226 2. Cuylen-Haering, S. et al. (2020) Nature 587, 285-290. PMID: 32879492 3. Yamazaki, H. et al. (2022) Nature cell biology 24, 625-632. PMID: 35513709 4. Hernandez-Armendariz, A. et al. (2024) Molecular cell 84, 3254-3270.e9. PMID: 39153474 | ||||||||
| 참고사항 | For research use only. |
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