Anti-LC3B Polyclonal Antibody (HV519014)

Name: Anti-LC3B Polyclonal Antibody
Brand: abinScience
SKU: HV519014
Price: 96 USD
Availability: InStock

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개요
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SDS

개요

카탈로그 번호

HV519014

설명

Anti-LC3B Polyclonal Antibody (HV519014) is a rabbit polyclonal antibody detecting MAP1LC3B in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human LC3B (Met1-Gly120).

타겟

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q9GZQ8, A6NCE7, O41515, Q9CQV6, Q62625

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Microtubule-associated protein 1 light chain 3 beta (LC3B/MAP1LC3B) is a ~14 kDa protein. Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

1. Weidberg, H. et al. (2010) The EMBO journal 29, 1792-802. PMID: 20418806

2. Zhu, Y. et al. (2013) The Journal of biological chemistry 288, 1099-113. PMID: 23209295

3. Wei, Y. et al. (2017) Cell 168, 224-238.e10. PMID: 28017329

4. Sentelle, RD. et al. (2012) Nature chemical biology 8, 831-8. PMID: 22922758

5. Tang, Z. et al. (2013) Nature 502, 254-7. PMID: 24089205

6. An, H. et al. (2019) Molecular cell 74, 891-908.e10. PMID: 31006537

7. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-LC3B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-LC3B Polyclonal Antibody (HV519014)

개요

이미지

참고 문헌

데이터시트

SDS

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