Anti-LEF1 Polyclonal Antibody (HV355014)

Name: Anti-LEF1 Polyclonal Antibody
Brand: abinScience
SKU: HV355014
Price: 96 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD): --

사양:

20ug
50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HV355014

설명

Anti-LEF1 Polyclonal Antibody (HV355014) is a rabbit polyclonal antibody detecting LEF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human LEF1 (Glu45-His260).

타겟

Lymphoid enhancer-binding factor 1, T cell-specific transcription factor 1-alpha, LEF-1, TCF1-alpha, LEF1

정제

Purified by antigen affinity column.

등록번호

Q9UJU2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Lymphoid enhancer-binding factor 1 (LEF1) is a ~44 kDa protein. Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.

1. Waterman, ML. et al. (1991) Genes & development 5, 656-69. PMID: 2010090

2. Brantjes, H. et al. (2001) Nucleic acids research 29, 1410-9. PMID: 11266540

3. Jesse, S. et al. (2010) International journal of cancer 126, 1109-20. PMID: 19653274

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-LEF1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: HepG2 cell lysate
Lane 3: BxPC3 cell lysate
Lane 4: K562 cell lysate
Lane 5: Mouse thymus lysate

참고 문헌

데이터시트

Anti-LEF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-LEF1 Polyclonal Antibody (HV355014)

개요

이미지

참고 문헌

데이터시트

SDS