Anti-LEP Polyclonal Antibody (HW360014)

Name: Anti-LEP Polyclonal Antibody
Brand: abinScience
SKU: HW360014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW360014

설명

Anti-LEP Polyclonal Antibody (HW360014) is a rabbit polyclonal antibody detecting LEP in ELISA, IHC, WB. Suitable for Human, Cat, Bovine, Bubalus bubalis, and Capra hircus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human LEP (Val22-Cys167).

타겟

OBS, OB, Obese protein, LEP, Leptin, Obesity factor

정제

Purified by antigen affinity column.

등록번호

P41159

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Leptin is a ~18 kDa protein. Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides.

1. Otero, M. et al. (2005) Arthritis research & therapy 7, R581-91. PMID: 15899045

2. Saxena, NK. et al. (2007) The Journal of biological chemistry 282, 13316-25. PMID: 17344214

3. Vuolteenaho, K. et al. (2009) Mediators of inflammation 2009, 345838. PMID: 19688109

4. Park, HY. et al. (2001) Experimental & molecular medicine 33, 95-102. PMID: 11460888

5. El-Zein, O. et al. (2013) PloS one 8, e83360. PMID: 24340098

6. Cassano, S. et al. (2014) Metabolism: clinical and experimental 63, 1272-9. PMID: 25060689

7. Chehab, FF. et al. (1996) Nature genetics 12, 318-20. PMID: 8589726

8. Jiang, H. et al. (2008) Biochemical and biophysical research communications 368, 1-5. PMID: 18242580

9. Zhao, Y. et al. (2003) Biochemical and biophysical research communications 300, 247-52. PMID: 12504075

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-LEP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-LEP Polyclonal Antibody (HW360014)

개요

이미지

참고 문헌

데이터시트

SDS

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