Anti-LIPE Polyclonal Antibody (HC681014)

Name: Anti-LIPE Polyclonal Antibody
Brand: abinScience
SKU: HC681014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HC681014

설명

Anti-LIPE Polyclonal Antibody (HC681014) is a rabbit polyclonal antibody detecting LIPE in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human LIPE (Met302-Leu425).

타겟

Monoacylglycerol lipase LIPE, LIPE, HSL, Retinyl ester hydrolase, REH, Hormone-sensitive lipase

정제

Purified by antigen affinity column.

등록번호

Q05469

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Hormone-sensitive lipase (LIPE) is a ~116 kDa protein. Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

1. Ali, YB. et al. (2005) Journal of lipid research 46, 994-1000. PMID: 15716583

2. Gao, J. et al. (2005) The Journal of investigative dermatology 124, 1259-66. PMID: 15955102

3. Rodriguez, JA. et al. (2010) Biochimica et biophysica acta 1801, 77-83. PMID: 19800417

4. Holst, LS. et al. (1996) Genomics 35, 441-7. PMID: 8812477

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-LIPE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-LIPE Polyclonal Antibody (HC681014)

개요

이미지

참고 문헌

데이터시트

SDS

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