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Anti-MAFG Polyclonal Antibody (HT080014)

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개요
카탈로그 번호HT080014
설명
Anti-MAFG Polyclonal Antibody (HT080014) is a rabbit polyclonal antibody detecting MAFG in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Chicken.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human MAFG (Met1-Ser162).
타겟 V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, Transcription factor MafG, MAFG, hMAF
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 O15525
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Transcription factor MafG is a ~17 kDa protein. Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NFE2L2. Activates globin gene expression when associated with NFE2L2.

1. Hung, HL. et al. (2001) The Journal of biological chemistry 276, 10715-21. PMID: 11154691
2. Johnsen, O. et al. (1996) Nucleic acids research 24, 4289-97. PMID: 8932385
3. Johnsen, O. et al. (1998) Nucleic acids research 26, 512-20. PMID: 9421508
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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