Anti-MAPK8 Polyclonal Antibody (HW707014)

Name: Anti-MAPK8 Polyclonal Antibody
Brand: abinScience
SKU: HW707014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HW707014

설명

Anti-MAPK8 Polyclonal Antibody (HW707014) is a rabbit polyclonal antibody detecting MAPK8 in ELISA, IHC, WB. Suitable for Cyprinus carpio, Danio rerio, Chicken, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human MAPK8 (Tyr26-Ile321).

타겟

MAP kinase 8, JNK1, SAPK1C, Stress-activated protein kinase 1c, Mitogen-activated protein kinase 8, SAPK1, c-Jun N-terminal kinase 1, SAPK1c, MAPK8, Stress-activated protein kinase JNK1, PRKM8, JNK-46, MAPK 8

정제

Purified by antigen affinity column.

등록번호

P45983

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Mitogen-activated protein kinase 8 (MAPK8) is a ~48 kDa protein. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.

1. Song, N. et al. (2017) Molecular cell 68, 185-197.e6. PMID: 28943315

2. Murata, T. et al. (2008) Analytical biochemistry 376, 115-21. PMID: 18307971

3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198

4. Tomlinson, V. et al. (2010) Cell death & disease 1, e29. PMID: 21364637

5. Deng, H. et al. (2011) The international journal of biochemistry & cell biology 43, 409-15. PMID: 21095239

6. Wei, Y. et al. (2008) Molecular cell 30, 678-88. PMID: 18570871

7. Hu, Y. et al. (2006) Molecular and cellular biology 26, 3282-94. PMID: 16581800

8. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

9. Nasrin, N. et al. (2009) PloS one 4, e8414. PMID: 20027304

10. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-JNK polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate

참고 문헌

데이터시트

Anti-MAPK8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-MAPK8 Polyclonal Antibody (HW707014)

개요

이미지

참고 문헌

데이터시트

SDS

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