Anti-MCM2 Polyclonal Antibody (HW401014)

Name: Anti-MCM2 Polyclonal Antibody
Brand: abinScience
SKU: HW401014
Price: 168 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

50ug
100ug

수량:

문의하기

개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW401014

설명

Anti-MCM2 Polyclonal Antibody (HW401014) is a rabbit polyclonal antibody detecting MCM2 in ELISA, IHC, WB. Suitable for Human, Mouse, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human MCM2 (Phe657-Phe904).

타겟

CCNL1, KIAA0030, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, CDCL1, BM28, DNA replication licensing factor MCM2

정제

Purified by antigen affinity column.

등록번호

P49736

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

DNA replication licensing factor MCM2 is a ~101 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

1. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

2. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

3. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

4. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

5. Todorov, IT. et al. (1994) Journal of cell science 107 ( Pt 1), 253-65. PMID: 8175912

6. Gao, J. et al. (2015) PloS one 10, e0133522. PMID: 26196677

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-MCM2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-MCM2 Polyclonal Antibody (HW401014)

개요

이미지

참고 문헌

데이터시트

SDS

빠른 접근

문의하기