Anti-Mouse CASP8 Polyclonal Antibody (MC266014)

Name: Anti-Mouse CASP8 Polyclonal Antibody
Brand: abinScience
SKU: MC266014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

MC266014

설명

Anti-Mouse CASP8 Polyclonal Antibody (MC266014) is a rabbit polyclonal antibody detecting Casp8 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Mouse CASP8 (Gly236-Pro480).

타겟

Caspase-8, CASP-8, 3.4.22.61, Caspase-8 subunit p18, Caspase-8 subunit p10, Casp8

정제

Purified by antigen affinity column.

등록번호

O89110

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/CD95 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Benchoua, A. et al. (2002) The Journal of biological chemistry 277, 34217-22. PMID: 12065591

2. Sun, H. et al. (2008) Cell 133, 415-26. PMID: 18455983

3. Orning, P. et al. (2018) Science (New York, N.Y.) 362, 1064-1069. PMID: 30361383

4. Sarhan, J. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E10888-E10897. PMID: 30381458

5. Newton, K. et al. (2019) Nature 574, 428-431. PMID: 31511692

6. Fritsch, M. et al. (2019) Nature 575, 683-687. PMID: 31748744

7. Muendlein, HI. et al. (2021) Nature communications 12, 86. PMID: 33397971

8. Luo, X. et al. (1998) Cell 94, 481-90. PMID: 9727491

9. Li, H. et al. (1998) Cell 94, 491-501. PMID: 9727492

10. Rickard, JA. et al. (2014) Cell 157, 1175-88. PMID: 24813849

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CASP8 polyclonal antibody at CASP8 on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: HCT116 cell lysate
Lane 4: THP-1 cell lysate

참고 문헌

데이터시트

Anti-Mouse CASP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse CASP8 Polyclonal Antibody (MC266014)

개요

이미지

참고 문헌

데이터시트

SDS

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