Anti-Mouse PD-1 Polyclonal Antibody (MS870014)

Name: Anti-Mouse PD-1 Polyclonal Antibody
Brand: abinScience
SKU: MS870014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

MS870014

설명

Anti-Mouse CD279/PDCD1/PD1 Polyclonal Antibody (MS870014) is a rabbit polyclonal antibody detecting CD279 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Mouse CD279/PDCD1/PD1 (Gly30-Glu146).

타겟

CD279, PD1, PDCD1, Programmed cell death protein 1, Protein PD-1, PD-1

내독소 수준

Please contact with the lab for this information.

순도

>90% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

Q02242

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Programmed cell death protein 1 (CD279/PD-1) is a ~31 kDa protein. Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival. CD279 is the therapeutic target of pembrolizumab (Keytruda) and nivolumab (Opdivo).

1. Nishimura, H. et al. (1999) Immunity 11, 141-51. PMID: 10485649

2. Nishimura, H. et al. (2001) Science (New York, N.Y.) 291, 319-22. PMID: 11209085

3. Okazaki, T. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 13866-71. PMID: 11698646

4. Okazaki, T. et al. (2011) The Journal of experimental medicine 208, 395-407. PMID: 21300912

5. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443

6. Latchman, Y. et al. (2001) Nature immunology 2, 261-8. PMID: 11224527

7. Lin, DY. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 3011-6. PMID: 18287011

8. Lázár-Molnár, E. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 10483-8. PMID: 18641123

9. Yokosuka, T. et al. (2012) The Journal of experimental medicine 209, 1201-17. PMID: 22641383

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Mouse PD-1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse PD-1 Polyclonal Antibody (MS870014)

개요

이미지

참고 문헌

데이터시트

SDS

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