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Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014)

Anti-Mouse HSPB1/HSP27 Polyclonal Antibody
Anti-Mouse HSPB1/HSP27 Polyclonal Antibody
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개요
카탈로그 번호MY322014
설명
Anti-Mouse HSPB1/HSP27 Polyclonal Antibody (MY322014) is a rabbit polyclonal antibody detecting HspB1 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Mouse HSPB1/HSP27 (Ala76-Pro174).
타겟 Heat shock protein beta-1, HspB1, Growth-related 25 kDa protein, Heat shock 25 kDa protein, HSP 25, Heat shock 27 kDa protein, HSP 27, p25, Hspb1, Hsp25, Hsp27
정제 Purified by antigen affinity column.
등록번호 P14602
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Heat shock protein beta-1 (HSPB1/HSP27) is a ~23 kDa protein. Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.

1. Huang, L. et al. (2007) Genesis (New York, N.Y. : 2000) 45, 487-501. PMID: 17661394
참고사항 For research use only.
이미지
  • Anti-Mouse HSPB1/HSP27 Polyclonal Antibody

    Western blot

    Lane 1: MCF-7 cell lysate
    Lane 2: Mouse heart lysate
    Lane 3: Rat heart lysate
    Lane 4: Mouse skeletal muscle lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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