Anti-Mouse KISS1R Polyclonal Antibody (MV546014)

Name: Anti-Mouse KISS1R Polyclonal Antibody
Brand: abinScience
SKU: MV546014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호

MV546014

설명

Anti-Mouse KISS1R Polyclonal Antibody (MV546014) is a rabbit polyclonal antibody detecting KiSS-1 receptor; KiSS-1R; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; mOT7T175; Kisspeptins receptor; Metastin receptor; Kiss1r; Gpr54 in ELISA, IHC, WB. Suitable for Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant mouse KISS1R (Ala2-Trp43&Leu102-Lys116&His181-Ala203&Val284-Lys305).

타겟

KiSS-1 receptor; KiSS-1R; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; mOT7T175; Kisspeptins receptor; Metastin receptor; Kiss1r; Gpr54

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q91V45

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

KiSS-1 receptor (KISS1R) is a ~43 kDa protein. Receptor for kisspeptins (kisspeptin-10, kisspeptin-13, kisspeptin-14 and metastin/kisspeptin-54). The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons. In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation. In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation.

1. Stafford, LJ. et al. (2002) Cancer research 62, 5399-404. PMID: 12359743

2. Seminara, SB. et al. (2003) The New England journal of medicine 349, 1614-27. PMID: 14573733

3. Funes, S. et al. (2003) Biochemical and biophysical research communications 312, 1357-63. PMID: 14652023

4. Messager, S. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1761-6. PMID: 15665093

5. Zhang, C. et al. (2008) The Journal of neuroscience : the official journal of the Society for Neuroscience 28, 4423-34. PMID: 18434521

6. Liu, X. et al. (2008) Endocrinology 149, 4605-14. PMID: 18483150

7. Ahow, M. et al. (2014) Endocrinology 155, 4433-46. PMID: 25147978

8. Li, Z. et al. (2024) Nature communications 15, 1300. PMID: 38346942

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Mouse KISS1R Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse KISS1R Polyclonal Antibody (MV546014)

개요

이미지

참고 문헌

데이터시트

SDS