Anti-Mouse METTL3 Polyclonal Antibody (MV489014)

Name: Anti-Mouse METTL3 Polyclonal Antibody
Brand: abinScience
SKU: MV489014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

MV489014

설명

Anti-Mouse METTL3 Polyclonal Antibody (MV489014) is a rabbit polyclonal antibody detecting METTL3 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Mouse METTL3 (Asp366-Leu580).

타겟

N6-adenosine-methyltransferase subunit METTL3, 2.1.1.348, Methyltransferase-like protein 3, N6-adenosine-methyltransferase 70 kDa subunit, MT-A70, Mettl3, Mta70

정제

Purified by antigen affinity column.

등록번호

Q8C3P7

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

N(6)-adenosine-methyltransferase catalytic subunit METTL3 is a ~64 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs.

1. Wang, Y. et al. (2014) Nature cell biology 16, 191-8. PMID: 24394384

2. Batista, PJ. et al. (2014) Cell stem cell 15, 707-19. PMID: 25456834

3. Geula, S. et al. (2015) Science (New York, N.Y.) 347, 1002-6. PMID: 25569111

4. Li, HB. et al. (2017) Nature 548, 338-342. PMID: 28792938

5. Xu, K. et al. (2017) Cell research 27, 1100-1114. PMID: 28809392

6. Zhang, C. et al. (2017) Nature 549, 273-276. PMID: 28869969

7. Yoon, KJ. et al. (2017) Cell 171, 877-889.e17. PMID: 28965759

8. Ke, S. et al. (2017) Genes & development 31, 990-1006. PMID: 28637692

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Mouse METTL3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse METTL3 Polyclonal Antibody (MV489014)

개요

이미지

참고 문헌

데이터시트

SDS