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| 카탈로그 번호 | MV568014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Mouse MLKL Polyclonal Antibody (MV568014) is a rabbit polyclonal antibody detecting Mixed lineage kinase domain-like protein; Mlkl in ELISA, IHC, WB. Suitable for Mouse.
Highlights
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| 종 반응성 | Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant mouse MLKL (Asp180-Val472). | ||||||||
| 타겟 | Mixed lineage kinase domain-like protein; Mlkl | ||||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q9D2Y4 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Mixed lineage kinase domain-like protein (MLKL) is a ~54 kDa protein. Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Does not have protein kinase activity. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function. 1. Wu, J. et al. (2013) Cell research 23, 994-1006. PMID: 23835476 2. Murphy, JM. et al. (2013) Immunity 39, 443-53. PMID: 24012422 3. Kaiser, WJ. et al. (2013) The Journal of biological chemistry 288, 31268-79. PMID: 24019532 4. Nogusa, S. et al. (2016) Cell host & microbe 20, 13-24. PMID: 27321907 5. Zhang, T. et al. (2020) Cell 180, 1115-1129.e13. PMID: 32200799 6. Jiao, H. et al. (2020) Nature 580, 391-395. PMID: 32296175 | ||||||||
| 참고사항 | For research use only |
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