Anti-Mouse PPIA Polyclonal Antibody (MX128014)

Name: Anti-Mouse PPIA Polyclonal Antibody
Brand: abinScience
SKU: MX128014
Price: 168 USD
Availability: InStock

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사양:

50ug
100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

MX128014

설명

Anti-Mouse PPIA Polyclonal Antibody (MX128014) is a rabbit polyclonal antibody detecting Ppia. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Mouse PPIA (Met1-Leu164).

타겟

Cyclophilin A, Cyclosporin A-binding protein, EC:5.2.1.8, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, Ppia, Rotamase A, SP18

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P17742

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Peptidyl-prolyl cis-trans isomerase A (PPIA) is a ~17 kDa protein. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2.

1. Wang, L. et al. (2014) Thrombosis and haemostasis 111, 873-82. PMID: 24429998

2. Lauranzano, E. et al. (2015) Brain : a journal of neurology 138, 974-91. PMID: 25678563

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-PPIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: K562 cell lysate
Lane 4: Raji cell lysate
Lane 5: Jurkat cell lysate
Lane 6: PC-12 cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

참고 문헌

데이터시트

Anti-Mouse PPIA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse PPIA Polyclonal Antibody (MX128014)

개요

이미지

참고 문헌

데이터시트

SDS