Anti-Mouse RIPK1 Polyclonal Antibody (MC317014)

Name: Anti-Mouse RIPK1 Polyclonal Antibody
Brand: abinScience
SKU: MC317014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

MC317014

설명

Anti-Mouse RIPK1 Polyclonal Antibody (MC317014) is a rabbit polyclonal antibody detecting Ripk1 in ELISA, IHC, WB. Suitable for Mouse and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Mouse RIPK1 (Met1-Pro310).

타겟

Receptor-interacting serine/threonine-protein kinase 1, 2.7.11.1, Cell death protein RIP, Receptor-interacting protein 1, RIP-1, Ripk1, Rinp, Rip

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q60855

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) is a ~74 kDa protein. Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway. Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis.

1. Newton, K. et al. (2014) Science (New York, N.Y.) 343, 1357-60. PMID: 24557836

2. Rickard, JA. et al. (2014) Cell 157, 1175-88. PMID: 24813849

3. Dillon, CP. et al. (2014) Cell 157, 1189-202. PMID: 24813850

4. Lin, J. et al. (2016) Nature 540, 124-128. PMID: 27819681

5. Geng, J. et al. (2017) Nature communications 8, 359. PMID: 28842570

6. Newton, K. et al. (2019) Nature 574, 428-431. PMID: 31511692

7. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280

8. Lalaoui, N. et al. (2020) Nature 577, 103-108. PMID: 31827281

9. Muendlein, HI. et al. (2021) Nature communications 12, 86. PMID: 33397971

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-RIPK1 polyclonal antibody at RIPK1 on various samples.
Lane 1: Rat heart lysate

참고 문헌

데이터시트

Anti-Mouse RIPK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Mouse RIPK1 Polyclonal Antibody (MC317014)

개요

이미지

참고 문헌

데이터시트

SDS

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