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| 카탈로그 번호 | MB941013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse, Rat, Rabbit | ||||
| 응용 분야 | Blocking, ELISA, FCM | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a, kappa | ||||
| 클론 ID | G6 | ||||
| 테스트된 응용 분야 | WB, FCM | ||||
| 타겟 | L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor | ||||
| 내독소 수준 | < 10 EU/mg | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | Q00731 | ||||
| RRID | Anti-Mouse VEGFA Antibody (G6) (abinScience Cat# MB941013, RRID:AB_3727197) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||
| 배경 | Vascular endothelial growth factor A, long form (VEGFA) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA is the therapeutic target of bevacizumab (Avastin). 1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969 | ||||
| 참고사항 | For research use only. | ||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Detects Mouse VEGFA in indirect ELISAs.
SDS-PAGE for Anti-Mouse VEGFA Antibody (G6)
Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: MB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
Lane 2: negative control
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