中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | JN091014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody (JN091014) is a rabbit polyclonal antibody detecting cards in ELISA, IHC, WB.
Highlights
|
||||||||
| 종 반응성 | Mycoplasma pneumoniae | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Mycoplasma pneumoniae CARDS (Met1-Phe591). | ||||||||
| 타겟 | ADP-ribosylating toxin CARDS, 2.4.2.-, ADP-ribosyltransferase CARDS, Community-Acquired Respiratory Distress Syndrome Toxin, CARDX TX, cards, MPN_372, MP464, Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P75409 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||||||
| 제품 사용 정보 |
| ||||||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | ADP-ribosylating toxin CARDS is a ~68 kDa protein. The main virulence factor for this bacteria, a mono-ADP-ribosylating toxin (mART), that transfers the ADP-ribosyl group from NAD(+) to multiple target proteins in vitro. Also elicits cytopathic effects in mammalian cells, such as disorganization and disruption of respiratory epithelial integrity in tracheal epithelium and vacuolization in the cytoplasm of CHO and HeLa cells as well as in mice and baboons. Treatment of mice or baboons with CARDS elicits a response that is consistent with human M.pneumoniae infections and mouse models of both infection and intoxication, suggesting that CARDS toxin is sufficient to cause prolonged inflammatory responses and airway dysfunction. Treatment of baboons with CARDS induces a number of cytokines; G-CSF (40 fold), IL-1Ra (10 fold), IL-6 and IL-8 (333 and 100 fold, respectively), MIP-1a (5 fold), and RANTES (9 fold). Treatment of mice gives a similar response. 1. Kannan, TR. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6724-9. PMID: 16617115 2. Kannan, TR. et al. (2014) Molecular microbiology 93, 568-81. PMID: 24948331 3. Bose, S. et al. (2014) mBio 5. PMID: 25538194 4. Ramasamy, K. et al. (2018) mBio 9. PMID: 29362229 5. Balasubramanian, S. et al. (2019) Cellular microbiology 21, e13032. PMID: 30977272 6. Ramasamy, K. et al. (2021) Scientific reports 11, 11571. PMID: 34078958 7. Hardy, RD. et al. (2009) PloS one 4, e7562. PMID: 19859545 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-0108 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Mycoplasma pneumoniae CARDS (Catalog No: JN091012)
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
