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Anti-NARS1 Polyclonal Antibody (HT196014)

Anti-NARS1 Polyclonal Antibody
Anti-NARS1 Polyclonal Antibody
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  • 개요

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개요
카탈로그 번호HT196014
설명
Anti-NARS1 Polyclonal Antibody (HT196014) is a rabbit polyclonal antibody detecting NARS1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human NARS1 (Met1-Arg330).
타겟 Asparagine--tRNA ligase, cytoplasmic, NARS, NRS, Asparaginyl-tRNA synthetase, NARS1, Asparaginyl-tRNA synthetase 1, AsnRS
정제 Purified by antigen affinity column.
등록번호 O43776
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Asparagine--tRNA ligase, cytoplasmic (NARS1) is a ~62 kDa protein. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells.

1. Manole, A. et al. (2020) American journal of human genetics 107, 311-324. PMID: 32738225
2. Wang, L. et al. (2020) Nature communications 11, 4038. PMID: 32788587
3. Beaulande, M. et al. (1998) Nucleic acids research 26, 521-4. PMID: 9421509
4. Howard, OM. et al. (2002) The Journal of experimental medicine 196, 781-91. PMID: 12235211
5. Park, JS. et al. (2018) International journal of biological macromolecules 120, 835-845. PMID: 30171954
참고사항 For research use only.
이미지
  • Anti-NARS1 Polyclonal Antibody

    Western blot

    Lane 1: HepG2 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: LO2 cell lysate
    Lane 7: Jurkat cell lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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