Anti-NGB Polyclonal Antibody (HV356014)

Name: Anti-NGB Polyclonal Antibody
Brand: abinScience
SKU: HV356014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HV356014

설명

Anti-NGB Polyclonal Antibody (HV356014) is a rabbit polyclonal antibody detecting NGB in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NGB (Met1-Glu151).

타겟

NGB, Neuroglobin

정제

Purified by antigen affinity column.

등록번호

Q9NPG2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Neuroglobin (NGB) is a ~16 kDa protein. Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia. In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress.

1. Dewilde, S. et al. (2001) The Journal of biological chemistry 276, 38949-55. PMID: 11473128

2. Pesce, A. et al. (2003) Structure (London, England : 1993) 11, 1087-95. PMID: 12962627

3. Guimarães, BG. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1005-14. PMID: 24699645

4. Wakasugi, K. et al. (2003) The Journal of biological chemistry 278, 36505-12. PMID: 12860983

5. Tiso, M. et al. (2011) The Journal of biological chemistry 286, 18277-89. PMID: 21296891

6. Watanabe, S. et al. (2008) Biochemical and biophysical research communications 369, 695-700. PMID: 18302932

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-NGB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-NGB Polyclonal Antibody (HV356014)

개요

이미지

참고 문헌

데이터시트

SDS

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