Anti-NOD1 Polyclonal Antibody (HV077014)

Name: Anti-NOD1 Polyclonal Antibody
Brand: abinScience
SKU: HV077014
Price: 168 USD
Availability: InStock

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개요
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SDS

개요

카탈로그 번호

HV077014

설명

Anti-NOD1 Polyclonal Antibody (HV077014) is a rabbit polyclonal antibody detecting NOD1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human NOD1 (Ala612-Asp826).

타겟

NOD1, Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, CARD4

정제

Purified by antigen affinity column.

등록번호

Q9Y239

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is a ~107 kDa protein. Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses. Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria. Preferentially binds iE-DAP in tripeptide-containing muropeptides (MurNAc-TriDAP or TriDAP). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2. Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling.

1. Inohara, N. et al. (2001) The Journal of biological chemistry 276, 2551-4. PMID: 11058605

2. Girardin, SE. et al. (2003) Science (New York, N.Y.) 300, 1584-7. PMID: 12791997

3. Chamaillard, M. et al. (2003) Nature immunology 4, 702-7. PMID: 12796777

4. Tanabe, T. et al. (2004) The EMBO journal 23, 1587-97. PMID: 15044951

5. Girardin, SE. et al. (2005) The Journal of biological chemistry 280, 38648-56. PMID: 16172124

6. Fukazawa, A. et al. (2008) PLoS pathogens 4, e1000228. PMID: 19043560

7. Lautz, K. et al. (2012) Cellular microbiology 14, 1568-83. PMID: 22672233

8. Vegna, S. et al. (2016) Journal of virology 90, 6022-6035. PMID: 27099311

10. Magalhaes, JG. et al. (2005) EMBO reports 6, 1201-7. PMID: 16211083

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-NOD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-NOD1 Polyclonal Antibody (HV077014)

개요

이미지

참고 문헌

데이터시트

SDS

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