Anti-OGT Polyclonal Antibody (HT047014)

Name: Anti-OGT Polyclonal Antibody
Brand: abinScience
SKU: HT047014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HT047014

설명

Anti-OGT Polyclonal Antibody (HT047014) is a rabbit polyclonal antibody detecting OGT in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human OGT (Lys177-Arg526).

타겟

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, OGT, O-linked N-acetylglucosamine transferase 110 kDa subunit, O-GlcNAc transferase subunit p110

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

O15294

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT) is a ~116 kDa protein. Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, AMPK, ATG4B, CAPRIN1, EZH2, FNIP1, GSDMD, KRT7, LMNA, LMNB1, LMNB2, RPTOR, HOXA1, PFKL, KMT2E/MLL5, MAPT/TAU, TET2, RBL2, RET, NOD2 and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity.

1. Yang, X. et al. (2002) Cell 110, 69-80. PMID: 12150998

2. Jínek, M. et al. (2004) Nature structural & molecular biology 11, 1001-7. PMID: 15361863

3. Liu, F. et al. (2009) Brain : a journal of neurology 132, 1820-32. PMID: 19451179

4. Whelan, SA. et al. (2010) The Journal of biological chemistry 285, 5204-11. PMID: 20018868

5. Lazarus, MB. et al. (2011) Nature 469, 564-7. PMID: 21240259

6. Daou, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 2747-52. PMID: 21285374

7. Lazarus, MB. et al. (2012) Nature chemical biology 8, 966-8. PMID: 23103939

8. Pathak, S. et al. (2015) Nature structural & molecular biology 22, 744-750. PMID: 26237509

9. Hou, CW. et al. (2016) Glycobiology 26, 13-8. PMID: 26369908

10. Ding, X. et al. (2015) PloS one 10, e0145023. PMID: 26678539

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-OGT Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-OGT Polyclonal Antibody (HT047014)

개요

이미지

참고 문헌

데이터시트

SDS