Anti-OPA1 Polyclonal Antibody (HT271014)

Name: Anti-OPA1 Polyclonal Antibody
Brand: abinScience
SKU: HT271014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HT271014

설명

Anti-OPA1 Polyclonal Antibody (HT271014) is a rabbit polyclonal antibody detecting OPA1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human OPA1 (Ser607-Lys960).

타겟

KIAA0567, OPA1, Optic atrophy protein 1, Dynamin-like 120 kDa protein, mitochondrial

정제

Purified by antigen affinity column.

등록번호

O60313

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Dynamin-like GTPase OPA1, mitochondrial is a ~111 kDa protein. Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain function. Exists in two forms: the transmembrane, long form (Dynamin-like GTPase OPA1, long form; L-OPA1), which is tethered to the inner mitochondrial membrane, and the short soluble form (Dynamin-like GTPase OPA1, short form; S-OPA1), which results from proteolytic cleavage and localizes in the intermembrane space. Both forms (L-OPA1 and S-OPA1) cooperate to catalyze the fusion of the mitochondrial inner membrane. The equilibrium between L-OPA1 and S-OPA1 is essential: excess levels of S-OPA1, produced by cleavage by OMA1 following loss of mitochondrial membrane potential, lead to an impaired equilibrium between L-OPA1 and S-OPA1, inhibiting mitochondrial fusion. The balance between L-OPA1 and S-OPA1 also influences cristae shape and morphology.

1. Ishihara, N. et al. (2006) The EMBO journal 25, 2966-77. PMID: 16778770

2. Song, Z. et al. (2007) The Journal of cell biology 178, 749-55. PMID: 17709429

3. Ban, T. et al. (2010) Human molecular genetics 19, 2113-22. PMID: 20185555

4. Anand, R. et al. (2014) The Journal of cell biology 204, 919-29. PMID: 24616225

5. Ban, T. et al. (2017) Nature cell biology 19, 856-863. PMID: 28628083

6. Huang, G. et al. (2017) Cell reports 20, 923-934. PMID: 28746876

7. Ge, Y. et al. (2020) eLife 9. PMID: 31922487

8. Zhang, D. et al. (2020) eLife 9. PMID: 32228866

9. Stephan, T. et al. (2020) The EMBO journal 39, e104105. PMID: 32567732

10. Hu, C. et al. (2020) Cell death & disease 11, 940. PMID: 33130824

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-OPA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-OPA1 Polyclonal Antibody (HT271014)

개요

이미지

참고 문헌

데이터시트

SDS

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