Anti-PAK1 Polyclonal Antibody (HC494014)

Name: Anti-PAK1 Polyclonal Antibody
Brand: abinScience
SKU: HC494014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC494014

설명

Anti-PAK1 Polyclonal Antibody (HC494014) is a rabbit polyclonal antibody detecting PAK1 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PAK1 (Glu252-Cys501).

타겟

Alpha-PAK, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, PAK1

정제

Purified by antigen affinity column.

등록번호

Q13153

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Serine/threonine-protein kinase PAK 1 (PAK1) is a ~60 kDa protein. Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases.

1. Zenke, FT. et al. (1999) The Journal of biological chemistry 274, 32565-73. PMID: 10551809

2. Manabe, R. et al. (2002) Journal of cell science 115, 1497-510. PMID: 11896197

3. Slack-Davis, JK. et al. (2003) The Journal of cell biology 162, 281-91. PMID: 12876277

4. Zhou, GL. et al. (2003) Molecular and cellular biology 23, 8058-69. PMID: 14585966

5. Zhou, H. et al. (2005) The Journal of biological chemistry 280, 10624-35. PMID: 15611088

6. Rider, L. et al. (2007) The Journal of biological chemistry 282, 30985-96. PMID: 17726028

7. Mayhew, MW. et al. (2007) Journal of cell science 120, 3911-8. PMID: 17989089

8. Harms, FL. et al. (2018) American journal of human genetics 103, 579-591. PMID: 30290153

9. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

10. Brown, JL. et al. (1996) Current biology : CB 6, 598-605. PMID: 8805275

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-PAK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-PAK1 Polyclonal Antibody (HC494014)

개요

이미지

참고 문헌

데이터시트

SDS

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