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| 카탈로그 번호 | HT315013 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Mouse | ||||
| 응용 분야 | IHC, WB, IF | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a, kappa | ||||
| 클론 ID | N136/16 | ||||
| 타겟 | Parkin, PARK2, Parkinson juvenile disease protein 2, E3 ubiquitin-protein ligase parkin, Parkin RBR E3 ubiquitin-protein ligase, PRKN, Parkinson disease protein 2 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | Q9WVS6 | ||||
| RRID | Anti-Parkin Antibody (N136/16) (abinScience Cat# HT315013, RRID:AB_3728020) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | E3 ubiquitin-protein ligase parkin (PRKN) is a ~51 kDa protein. Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. 1. Wang, C. et al. (2018) Nature communications 9, 81. PMID: 29311685 2. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033 3. Shiba-Fukushima, K. et al. (2014) PLoS genetics 10, e1004861. PMID: 25474007 4. Kuroda, Y. et al. (2012) Human molecular genetics 21, 991-1003. PMID: 22082830 5. Yun, J. et al. (2014) eLife 3, e01958. PMID: 24898855 | ||||
| 참고사항 | For research use only. | ||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
SDS-PAGE for Anti-Parkin Antibody (N136/16).
Mouse tissue section stained by immunofluorescence showing Parkin protein (green) in scattered cells, with nuclei counterstained by DAPI (blue).
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