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| 카탈로그 번호 | HF535014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-PARN Polyclonal Antibody (HF535014) is a rabbit polyclonal antibody detecting PARN in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human PARN (Lys178-Asp245). | ||||||||
| 타겟 | Deadenylating nuclease, Polyadenylate-specific ribonuclease, Poly(A)-specific ribonuclease PARN, Deadenylation nuclease, PARN, DAN | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | O95453 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Poly(A)-specific ribonuclease PARN is a ~73 kDa protein. 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. 1. Gao, M. et al. (2000) Molecular cell 5, 479-88. PMID: 10882133 2. Martînez, J. et al. (2001) The Journal of biological chemistry 276, 27923-9. PMID: 11359775 3. Lai, WS. et al. (2003) Molecular and cellular biology 23, 3798-812. PMID: 12748283 4. Gherzi, R. et al. (2004) Molecular cell 14, 571-83. PMID: 15175153 5. Körner, CG. et al. (1998) The EMBO journal 17, 5427-37. PMID: 9736620 6. Berndt, H. et al. (2012) RNA (New York, N.Y.) 18, 958-72. PMID: 22442037 7. Boele, J. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 11467-72. PMID: 25049417 | ||||||||
| 참고사항 | For research use only. |
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