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| 카탈로그 번호 | HY337013 | ||||||
|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||
| 응용 분야 | ELISA, WB | ||||||
| 숙주 종 | Human | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | IgG1, kappa | ||||||
| 클론 ID | SAA2241 | ||||||
| 타겟 | PCNA | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >90% as determined by SDS-PAGE. | ||||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||||
| 등록번호 | P12004 | ||||||
| RRID | Anti-PCNA Antibody (SAA2241) (abinScience Cat# HY337013, RRID:AB_3729877) |
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| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 대체 이름 | Cyclin, Proliferating cell nuclear antigen, PCNA | ||||||
| 배경 | DNA sliding clamp PCNA is a ~28 kDa protein. Confers DNA tethering and processivity to DNA polymerases and other proteins. Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of DNA replication by increasing the polymerases' processivity during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. 1. Nicolae, CM. et al. (2014) The Journal of biological chemistry 289, 13627-37. PMID: 24695737 2. Cazzalini, O. et al. (2014) Nucleic acids research 42, 8433-48. PMID: 24939902 3. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232 | ||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: MCF-7 cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse spleen lysate
Lane 9: Rat spleen lysate
Western blot analysis was performed using anti-PCNA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PCNA (Catalog No: HY337012)
Lane 2: negative control
Detects recombinant human PCNA (Catalog No: HY337012) in indirect ELISA
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