Anti-PDK1 Polyclonal Antibody (HS822014)

Name: Anti-PDK1 Polyclonal Antibody
Brand: abinScience
SKU: HS822014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HS822014

설명

Anti-PDK1 Polyclonal Antibody (HS822014) is a rabbit polyclonal antibody detecting PDK1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PDK1 (Tyr233-Met430).

타겟

PDHK1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

정제

Purified by antigen affinity column.

등록번호

Q15118

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) is a ~49 kDa protein. Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia.

1. Gudi, R. et al. (1995) The Journal of biological chemistry 270, 28989-94. PMID: 7499431

2. McFate, T. et al. (2008) The Journal of biological chemistry 283, 22700-8. PMID: 18541534

3. Hitosugi, T. et al. (2011) Molecular cell 44, 864-77. PMID: 22195962

4. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675

5. Kato, M. et al. (2007) Structure (London, England : 1993) 15, 992-1004. PMID: 17683942

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-PDK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: 293T cell lysate
Lane 3: PC-12 cell lysate
Lane 4: Lncap cell lystae
Lane 5: Hela cell lysate

참고 문헌

데이터시트

Anti-PDK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-PDK1 Polyclonal Antibody (HS822014)

개요

이미지

참고 문헌

데이터시트

SDS