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Anti-PDXK Polyclonal Antibody (HT485014)

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개요
카탈로그 번호HT485014
설명
Anti-PDXK Polyclonal Antibody (HT485014) is a rabbit polyclonal antibody detecting PDXK in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Ovis aries, and Pig.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human PDXK (Gln29-Lys253).
타겟 Pyridoxine kinase, Pyridoxal kinase, C21orf97, PDXK, PNK, PKH, C21orf124
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 O00764
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Pyridoxal kinase (PDXK) is a ~35 kDa protein. Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.

1. Lee, HS. et al. (2000) Molecules and cells 10, 452-9. PMID: 10987144
2. Musayev, FN. et al. (2007) Protein science : a publication of the Protein Society 16, 2184-94. PMID: 17766369
3. Gandhi, AK. et al. (2009) Biochemical and biophysical research communications 381, 12-5. PMID: 19351586
4. Chelban, V. et al. (2019) Annals of neurology 86, 225-240. PMID: 31187503
5. Hanna, MC. et al. (1997) The Journal of biological chemistry 272, 10756-60. PMID: 9099727
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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