Anti-PHF2 Polyclonal Antibody (HB551014)

Name: Anti-PHF2 Polyclonal Antibody
Brand: abinScience
SKU: HB551014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HB551014

설명

Anti-PHF2 Polyclonal Antibody (HB551014) is a rabbit polyclonal antibody detecting Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant human PHF2 (Gln83-Arg450).

타겟

Lysine-specific demethylase PHF2; 1.14.11.-; GRC5; PHD finger protein 2; PHF2; CENP-35; KIAA0662

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

O75151, Q9WTU0

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Lysine-specific demethylase PHF2 is a ~120 kDa protein. Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

1. Wen, H. et al. (2010) The Journal of biological chemistry 285, 9322-9326. PMID: 20129925

2. Horton, JR. et al. (2011) Journal of molecular biology 406, 1-8. PMID: 21167174

3. Baba, A. et al. (2011) Nature cell biology 13, 668-75. PMID: 21532585

참고사항

For research use only

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참고 문헌

데이터시트

Anti-PHF2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-PHF2 Polyclonal Antibody (HB551014)

개요

이미지

참고 문헌

데이터시트

SDS