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| 카탈로그 번호 | HV460023 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | IHC, IP, WB | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a, kappa | ||||
| 클론 ID | P3.28 | ||||
| 타겟 | RIPK3, RIP-like protein kinase 3, RIP3, Receptor-interacting serine/threonine-protein kinase 3, RIP-3, Receptor-interacting protein 3 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | Q9Y572 | ||||
| RRID | Anti-Phospho-RIP3 (pS199) Antibody (P3.28) (abinScience Cat# HV460023, RRID:AB_3725739) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | Receptor-interacting serine/threonine-protein kinase 3 (p-RIPK3) is a ~56 kDa protein. Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. 1. He, S. et al. (2009) Cell 137, 1100-11. PMID: 19524512 2. Cho, YS. et al. (2009) Cell 137, 1112-23. PMID: 19524513 3. Sun, L. et al. (2012) Cell 148, 213-27. PMID: 22265413 4. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414 5. Zhao, J. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 5322-7. PMID: 22421439 6. Choi, SW. et al. (2018) Molecular cell 70, 920-935.e7. PMID: 29883609 7. Ashida, H. et al. (2020) The EMBO journal 39, e104469. PMID: 32657447 8. Zheng, M. et al. (2020) Cell 181, 674-687.e13. PMID: 32298652 | ||||
| 참고사항 | For research use only. | ||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
SDS-PAGE for Anti-Phospho-RIP3 (pS199) Antibody (P3.28).
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