中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HF948014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-PIGR Polyclonal Antibody (HF948014) is a rabbit polyclonal antibody detecting PIgR in ELISA, IHC, WB. Suitable for Capra hircus and Human.
Highlights
|
||||||||
| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human PIGR (Arg289-Arg638). | ||||||||
| 타겟 | Poly-Ig receptor, Polymeric immunoglobulin receptor, PIgR, PIGR, Hepatocellular carcinoma-associated protein TB6 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P01833 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||||||
| 제품 사용 정보 |
| ||||||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Polymeric immunoglobulin receptor (PIGR) is a ~83 kDa protein. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment. | ||||||||
| 참고사항 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting PIGR (HF948014) at a concentration of 16 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of PIGR was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-PIGR polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: HL60 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MOLT-4 cell lysate
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
