Anti-PLA2G2A Polyclonal Antibody (HB944014)

Name: Anti-PLA2G2A Polyclonal Antibody
Brand: abinScience
SKU: HB944014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HB944014

설명

Anti-PLA2G2A Polyclonal Antibody (HB944014) is a rabbit polyclonal antibody detecting PLA2G2A in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PLA2G2A (Asn21-Cys144).

타겟

Phosphatidylcholine 2-acylhydrolase 2A, NPS-PLA2, RASF-A, Non-pancreatic secretory phospholipase A2, Group IIA phospholipase A2, GIIC sPLA2, PLA2L, PLA2G2A, PLA2B, Phospholipase A2, membrane associated

정제

Purified by antigen affinity column.

등록번호

P14555

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Phospholipase A2, membrane associated (PLA2G2A) is a ~16 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes.

1. Ishizaki, J. et al. (1999) The Journal of biological chemistry 274, 24973-9. PMID: 10455175

2. Suzuki, N. et al. (2000) The Journal of biological chemistry 275, 5785-93. PMID: 10681567

3. Kramer, RM. et al. (1989) The Journal of biological chemistry 264, 5768-75. PMID: 2925633

4. Laine, VJ. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 162, 7402-8. PMID: 10358193

5. Koduri, RS. et al. (2002) The Journal of biological chemistry 277, 5849-57. PMID: 11694541

6. Boudreau, LH. et al. (2014) Blood 124, 2173-83. PMID: 25082876

7. Sun, YX. et al. (2004) The Biochemical journal 380, 749-56. PMID: 14998370

8. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536

9. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-PLA2G2A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-PLA2G2A Polyclonal Antibody (HB944014)

개요

이미지

참고 문헌

데이터시트

SDS