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Anti-POMT1 Polyclonal Antibody (HV576014)

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개요
카탈로그 번호HV576014
설명
Anti-POMT1 Polyclonal Antibody (HV576014) is a rabbit polyclonal antibody detecting POMT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human POMT1 (Pro318-His513).
타겟 POMT1, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, Protein O-mannosyl-transferase 1
정제 Purified by antigen affinity column.
등록번호 Q9Y6A1
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Protein O-mannosyl-transferase 1 (POMT1) is a ~84 kDa protein. Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.

1. Beltrán-Valero de Bernabé, D. et al. (2002) American journal of human genetics 71, 1033-43. PMID: 12369018
2. Manya, H. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 500-5. PMID: 14699049
3. Larsen, ISB. et al. (2017) The Journal of biological chemistry 292, 11586-11598. PMID: 28512129
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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