Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody (JN089014)

Name: Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody
Brand: abinScience
SKU: JN089014
Price: 168 USD
Availability: InStock

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100ug

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

JN089014

종 반응성

Porphyromonas gingivalis

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Porphyromonas gingivalis kgp/prtK (Asp229-Pro683).

타겟

Lys-gingipain W83, 3.4.22.47, Lysine specific cysteine protease, Lysine-specific cysteine proteinase, Porphypain, PrtK48, Lys-gingipain catalytic subunit, 39 kDa adhesin, PrtK39, 15 kDa adhesin, PrtK15, 44 kDa adhesin, PrtK44, kgp, prtK, prtP

정제

Purified by antigen affinity column.

등록번호

Q51817

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Lys-gingipain W83 (kgp/prtK) is a ~187 kDa protein. Cysteine proteinase with a strong preference for substrates with Lys in the P1 position. Hydrolyzes bovine hemoglobin, bovine serum albumin, casein, human placental type I collagen and human IgA and IgG. Disrupts the functions of polymorphonuclear leukocytes. May act as a virulence factor in the development of peridontal disease. Involved in the coaggregation of P.gingivalis with other oral bacteria.

1. Bhogal, PS. et al. (1997) Microbiology (Reading, England) 143 ( Pt 7), 2485-2495. PMID: 9245829

2. Li, N. et al. (2011) Molecular microbiology 81, 1358-73. PMID: 21812842

3. Ganuelas, LA. et al. (2013) European journal of microbiology & immunology 3, 152-62. PMID: 24265933

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-Kgp polyclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 70 ug
Lane 2: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 50 ug
Lane 3: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 30 ug
Lane 4: recombinant Porphyromonas gingivalis kgp/prtK (Catalog No: JN089012), 10 ug

참고 문헌

데이터시트

Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Porphyromonas gingivalis kgp/prtK Polyclonal Antibody (JN089014)

개요

이미지

참고 문헌

데이터시트

SDS

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