Anti-PPARG Polyclonal Antibody (HW678014)

Name: Anti-PPARG Polyclonal Antibody
Brand: abinScience
SKU: HW678014
Price: 96 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HW678014

설명

Anti-PPARG Polyclonal Antibody (HW678014) is a rabbit polyclonal antibody detecting PPARG in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human PPARG (Asp230-Tyr505).

타겟

NR1C3, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, PPARG

정제

Purified by antigen affinity column.

등록번호

P37231

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Peroxisome proliferator-activated receptor gamma (PPARG) is a ~57 kDa protein. Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid metabolism, glucose homeostasis and beta-oxidation of fatty acids. Activated by lipid ligands: binds peroxisome proliferators, such as hypolipidemic drugs, and fatty acids, such as prostaglandin J2 metabolites. Ligand-binding results in a conformational change in the receptor, promoting dissociation of repressors and recruitment of coactivators, and subsequent activation of target gene expression. Specifically binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses.

1. Yin, Y. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 268-78. PMID: 16150867

2. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347

3. Katano-Toki, A. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 769-80. PMID: 23525231

4. Elbrecht, A. et al. (1996) Biochemical and biophysical research communications 224, 431-7. PMID: 8702406

5. Lambe, KG. et al. (1996) European journal of biochemistry 239, 1-7. PMID: 8706692

6. Mukherjee, R. et al. (1997) The Journal of biological chemistry 272, 8071-6. PMID: 9065481

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-PPARG Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-PPARG Polyclonal Antibody (HW678014)

개요

이미지

참고 문헌

데이터시트

SDS

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