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| 카탈로그 번호 | HA677014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-PRKCH Polyclonal Antibody (HA677014) is a rabbit polyclonal antibody detecting Protein kinase C eta type; 2.7.11.13; PKC-L; nPKC-eta; PRKCH; PKCL; PRKCL in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
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| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant human PRKCH (Arg349-Gln626). | ||||||||
| 타겟 | Protein kinase C eta type; 2.7.11.13; PKC-L; nPKC-eta; PRKCH; PKCL; PRKCL | ||||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P24723, P23298, Q64617 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Protein kinase C eta type (PRKCH) is a ~77 kDa protein. Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. | ||||||||
| 참고사항 | For research use only |
Western blot analysis was performed using anti-PRKCH polyclonal antibody at 1ug/mL on various samples.
Lane 1: MCF-7 cell lysate
Lane 2: Jurkat cell lysate
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